Grape seed extract( Polyphenol, proanthocyanidin)

→Research Report of Animal Experiment for

Formulation of Proanthocyanidin:

Dimer 　Trimer    Tetramer

Function and application:

The Grape Seed extract is a new type of efficient anti-oxidant, it has the strongest effect among all of the current free radicals clearance and it is very active inside the body.

Our company has applied ourselves in the research of Grape seed extract, make professional production process, and can now supply various specification of Grape seed extract as per customers requirement.

It is proved by the experiment that the anti-oxidant ability of OPC is 50 times than Vit.E, 20 times than Vit.C, also it can be quickly, completely absorbed.

The blood density can reach to the highest density after take it for 20 minutes and the half life of metabolizing is about 7 hours long.

In a word, the OPC has the following health care effect:

1, Lower the outbreak of the disease as: Heart disease, Cancer, premature senility, arthritis, Hypertension, dementia,diabetic.

2, Prevent over 70% disease which related to the free radicals, strengthen the wall of the blood vessel's resistibility, lower the brittleness of the capillary vessel and maintain its permeation, alleviate the swell and dropsy of the leg , buildup the capillary vessel, vein, artery's function, relief the damage through the sport, prevent and alleviate the varix, has the treatment of the damage and defection of Chronic vein, increase the flexible of the membrane of the red cells, buildup the skin's flexibility and softy, prevent the damage to the skin by the ultraviolet radiation.

About Allergic:

It is valid to the pollenosis (pollen allergy).

About Inflammation:

Anti-inflammation, increase the flexibility of the joint, alleviate the ache of the joint swell, relief the diabetes retinitis, prevent blindness which caused by it.

About Immune system: it can strengthen the immunity, lower the frequency of the flu, alleviate its seriousness,

About digestive:

It showed the obvious effect on the acute gastric ulcer which cause by the strain. it is also valid to the digestive inflammation.

Application and Dose:

1, the grape seed extract can be make into capsules as the raw material of the healthy food, and it is regarded as the first ten popular natural plant, possess the honor of the natural health care and the cosmetics.

2, The high quality OPC, cause its good solubility in the water and ethanol, plus its brilliance color, the curative effect, has been widely added into the beverage and the wine, cosmetics field as the functional content to make water or cream cosmetics, it has the function of anti-caducity and wipe off the spot and regarded as the newly arisen cosmetics.

3,As the natural functional content which possess the strong anti-oxidant, it is widely added into all kinds of cake, cheese as the nurture, natural antiseptic in Europe and USA, it has increased the safety of the food.

Products characteristics:

1,All kinds of specification of grape seed extract,(40%,60%,80%,90%,95%), high quality can meet different customer's requirement.

2,The raw material is adopt the excellent grape seed from the the north of Yellow River which is suitable for the grape's grow, it is free of pestiside.

3,The extraction process is only use water and ethanol, ensure the safety of the product.

4,The oligomers( ) content is very high, ensure the unit proanthocyanidin's treatment.

5, low ash, impurity, heavy metal residue is lower than 10ppm.

6,It has good solubility in water and ethanol, so it was widely used in different field.

The specification of the Grape seed extract:

Standard quality:

HPLC of

Soybean Isoflavones:

Soy isoflavones are powerful plant substances chemically similar to the female hormone estrogen. Their presence in soy foods may help to explain why people in countries where soy is a big part of the diet suffer from relatively few menopausal symptoms such as hot flashes. It may also explain why rates of certain hormone-related cancers are much lower in Asia than they are in many parts of the West, including the United States.

While soy isoflavones are now available in supplement form, it remains to be determined whether these pills can provide the same health benefits as soy isoflavones consumed as part of the diet. In fact, most research has been done on people who eat soy products rather than take soy supplements. It's possible that isoflavones are just one of several therapeutic compounds in soy. Still, while it's ideal to get soy isoflavones from one or two daily servings of soy foods, supplements are now an option for soy-leery eaters.

Specifically, soy isoflavones may help to:

Control symptoms of menopause and perimenopause;

Guard against osteoporosis;

Counteract the effects of endometriosis;

Protect against prostate problems;

Prevent various cancers;

Reduce heart disease risk.

Specification: 10% 20% 40% 80% 90%

HPLC of Soy Isoflavones

Apple Polyphenol:
Appjfnol is a natural blend of polyphenols with brown dried powder ; which extracted from apple skin, it has a hint of apple aroma and good solubility toward water and ethanol. This product also has a very stable quality even to heat ,acid and high adaptability to processing.

Appjfnol is distinct when compared to polyphenolic concentrates derived from other sources currently on the market. One of the most concentrated natural sources of polyphenols, Appjfnol contains a unique blend of procyanidins as the primary constituents. Clinical studies have shown that, in addition to its activity as a dietary antioxidant, strengthen the power of the muscle and reduce the content of the fat in the innards, Appjfnol also helps maintain respiratory health even during the allergy season, woks to whiten skin by limiting the formation of melanin and, has the good prevention of the skin to the ultraviolet radiation, for oral care, inhibit the halitosis caused by saliva during sleep and dental plaque.

Appjfnol processes its own laboratory and research, development department; which will demonstrate the performance of this product or we would also like very much to customize an application to suit your specific product requirement.

Contact the JF Natural to obtain more information about the Appjfnol , to know what's in it for you, once you have it, you will never find the same again.

HPLC of Appjfnol

Phloridzin:

Phloridzin (Phlorizin) is an anti-diabetic agent that is found primarily in apple peels. Phloridzin inhibits intestinal glucose uptake via the sodium D-glucose co-transporter and similarly inhibits renal glucose re-absorption. Correction of hyperglycemia with Phloridzin has been shown to normalize the effects of insulin on glucose metabolism in the liver and other peripheral tissues such as muscle and adipose tissue in diabetic rat models.β-Cell abnormalities were also completely corrected when diabetic rats were treated with Phloridzin to normalize plasma glucose levels. Obviously, based on its effect on mRNA levels, phloridzin is a genomeceutical.

Reference literature

1) Brichard, SM, Henquin, JC, Girard, J. Phlorizin treatment of diabetic rats partially reverses the abnormal expression of genes involved in hepatic glucose metabolism. Diabetologia. (1993) 36:292-298.
2) Goda T. Regulation of the expression of carbohydrate digestion/absorption-related genes. Br J Nutr. 2000 Dec;84 Suppl 2:S245-8.

Application Patent

Patent number: JP2002322067
PHLORIDZIN-RICH PHENOLIC FRACTION AND ITS USE AS COSMETIC, DIETARY CARE AGENT OR NUTRACEUTICAL AGENT
Inventor: GAUDOUT DAVID; MEGARD DENIS; (+3)
Applicant: DIANA INGREDIENTS
EC: A23L1/30B; A61K35/78; (+1)
IPC: A23L1/30; C07D311/62; A23L1/30 (+17)
Publication info: JP2002322067 - 2002-11-08
Abstract
PROBLEM TO BE SOLVED: To obtain a phenol fraction which can be used as a cosmetic, a dietary care agent or a nutraceutical agent and contains rich dihydrochalcones (phloridzin and phloretin) obtained from the fruits of a rosaceous plant, and to develop a method for obtaining the fraction. SOLUTION: The fraction obtained from the fruits of the rosaceous plant is characterized by containing at least 20 wt. % of polyphenols containing phloridzin in an amount of at least 10 wt. % based on the total phenols, and the method for preparing the fraction comprises starting from crushed apples, carrying out one or more solid/liquid extractions, drying the solid extract, and then extracting the dried product with an organic solvent.

Patent number: JP4235112
TYROSINASE-INHIBITOR
Publication date: 1992-08-24
Inventor: MITANI YASUMASA; SAKAI SHUNSUKE
Applicant: TEI EE SHII GIJIYUTSU KAGAKU K
Abstract
PURPOSE:To obtain a tyrosinase-inhibitor usable as a skin-beautifying agent for cosmetic and an agent for preventing blackening and browning of food, etc., by using 1,3,5-trihydroxybenzene derivative as an active component. CONSTITUTION:The active component of the objective tyrosinase-inhibitor is the compound of formula I (R1 is H or glucose residue; R2 is H or group of formula II ; at least one of R1 groups is H but all of R1 and R2 groups are not H at the same time), e.g. phlorin of formula III, phloridzin of formula IV or phloretin of formula V. These compounds have remarkable tyrosinase- inhibiting activity comparable or superior to that of known agent such as kojic acid and arbutin. The amount of the compound is preferably about 0.5-1g per 100g of cosmetic in the case of using as a skin-beautifying cosmetic or 1-2g per 100l of solution in the case of using as a blackening preventing agent for food preferably in the form of an aqueous solution or an organic solvent solution.

Rhodiola Rosea E.:

Rhodiola rosea is a popular plant in traditional medical systems in Eastern Europe and Asia with a reputation for stimulating the nervous system, decreasing depression, enhancing work performance, eliminating fatigue, and preventing high altitude sickness. Rhodiola rosea has been categorized as an adaptogen by Russian researchers due to its observed ability to increase resistance to a variety of chemical, biological, and physical stressors. Its claimed benefits include antidepressant, anticancer, cardioprotective, and central nervous system enhancement. Research also indicates great utility in asthenic conditions (decline in work performance, sleep difficulties, poor appetite, irritability, hypertension, headaches, and fatigue) developing subsequent to intense physical or intellectual strain. The adaptogenic, cardiopulmonary protective, and central nervous system activities of Rhodiola rosea have been attributed primarily to its ability to influence levels and activity of monoamines and opioid peptides such as beta-endorphins.

HPLC of Rhodiola rosea Extract

Serrate Clubmoss P.E.:

Huperzine A (Huperzia serrata) is an ingredient found in Chinese Moss that enhances mental functioning by prolonging the life of the brain neurotransmitter acetylcholine. It works by inhibiting acetylcholinesterase, the enzyme that breaks down acetylcholine.

Rose Flowers P.E.:
The precious active constituent is the repair tonic of cell and the tissue, has promotion of cell regeneration function, and is to be well known as micro vascular dilatation and skin astringent for moistening the skin. Application: Each kind of hair wash, bath, soap. Representing Brands: Biyoutefu, Yunhi, Sunmaster, Sarbo, Boss-in。

Panax Ginseng P.E.:
Contains rich vitamin B1, B2, Ginsenosides and Phytosterone, it may strengthen the skin tissue, promotes the skin tissue regeneration, enhances the tissue function, and prevented the skin aging process, eliminate fatigue. Application: Each kind of hair wash, bath, soap. Representing Brands: Givenchy, Yunhi, Biyoutefu, Clarins, Tjoy.

Polygonum Multiflorum P.E.:
Has blackening hair and anti aging function, can also promote the reaction of the SOD, dilates blood vessel, delays the cell regeneration process, inhibits the early senility of the skin. Application: Each kind of hair wash, bath, soap. Representing Brands: Rejoice, Olive, Boss-in and so on.

Rosemary P.E.:
Rosemary extract is high-efficient antioxidant, used in cosmetics can remove skin pigmentation, anti-oxidant, strengthen the skin elasticity, slowing aging etc., and has overcome the weakness of the instability of the SOD. Application: Various kinds of hair lotion, shower lotion. Representing Brands: Yunhi, Clarins, Vestalia and so on.

Arbutin:
The active substance originated from natural plant which can whiten and lighten skin. Using arbutin as active constituent of cosmetics to treat the skin pigmentation can have a prominent. Add in appropriate proportion to cosmetics, it has the good curative effect to the freckle and the butterfly spot as well as the skin pigmentation.

Chinese Flowering Quince P.E.:
The papayin may carry on the normal scaling process of dead surface skin makes the dead skin not causing aging and blocking the pore, prevents the scaring and the appear of wrinkle, makes the face skin to be exquisite and bright.

Fo-Ti P.E.:
Can promote the hair melanin production; improve the whitening of hair caused by trace element deficiency, the yellow and dryness of hair. After boiling, the red purple element dissolved in oil has a cosmetic function. Combined with other natural medicine or added to any kind of cosmetics has a function of stopping itching, preventing hair loss, blackening hair and enhancing the skin elasticity.

Resveratrol:
A kind plant source female hormone. It has a very good prevention function for acne (youth smallpox).

5-HTP

Functions: It has the function of Anti-hypochondria, Anti-epilepsy, Anti- Parkinson's disease , also can be used for weight loss, quitted the addiction ,improving sleeping ,effected on the relief of emmenia period's disease and cure the migraine.

Our products Characteristics:

1, the whole production process uses only water, ethanol, sour alkali, None use of any organism solvent.

2, Good water-solubility, wide used in different field.

Specification: 95%,98%,99% (Method: HPLC)

Shikimic acid

The shikimic acid is one kind of monomer compound shikimic acid which withdraws from the Chinese native medicine truestar anise tree has the obvious anti- thrombus to form the function, may suppress moves, the vein thrombus and the thrombosis and takes the anti- bird flu medicine "Duffy" the foundation raw material.

Specification: 98% 99%

Black Soybean Red Color
1.Function and Application
The extract is from the natural black soybean peel with dark-purplish red powder which has a good solubility in water-ethanol solution, the main active ingredient iscyanidin-3-galactoside, a efficient antioxidant, can be used widely in the healthy food industry.
It shows pink in acid solutions, purplish-red in neutral solutions and blue-black in alkaline solutions. According to GB-2760-1996, this product could be used in beverage, wine, food and cosmetics.
2. Quality and Specifications
a. Anthocyanin 5% 10% 25% UV
b. Color Value E1%1cm525±5nm：20 30 40 50 60 UV
3. Product characteristics
a. The extract solvent is only with water and ethanol; which ensure the safety of the product.
b. Stable techinic with strict quality control system.
c. Low ash, impurity.
d. Complete product tracing system and techinic support.
Lutein
1.Function and Application
Lutein is a natural pigment of the carotenoid family. It is widely found in nature, mainly in vegetables and fruits. In the human body, Lutein is found in tissues such as liver, pancreas, ovaries, and testicles and in the macula, lens and retina of the eye. Just like other carotenoid, lutein acts as an antioxidant, protecting cells against the damaging effects of free radicals and/or as a filter in light sensitive tissues such as the eye macula, lens and retina. Unlike ß-Carotene, lutein is not a Vitamin A precursor.
It is increasingly recognized as a key factor in maintaining the health of the macula, an area in the back of the eye's retina that creates the sharpest vision. Free radical damage from sunlight and other environmental factors generally results in the onset of Age-Related Macular Degeneration.
Lutein is not made in the human body and has to be obtained from food (preferably spinach, kale, collard, broccoli, and peas). Nowadays, Lutein is becoming an increasingly popular active ingredient used in the nutritional supplements industry.
2.Quality and Specifications
90% HPLC
3.Product characteristics
a. Low ash, impurity, heavy metal, solvent residue.
b. Technology steadily, quality controls strictly.
c. Complete product tracing system and techinic support.


Zeaxanthin
1.Function and Application
Zeaxanthin is less common in the human diet - coming from certain orange and yellow fruits and vegetables. Meso-zeaxanthin is not found in the normal human diet, and is thought to originate from lutein. The high concentrations and specific uptake of the macular carotenoids is mediated by specific binding proteins that draw them into the tissue and stabilize them.
Studies have shown that eating lutein- and zeaxanthin-rich foods or taking supplements of these carotenoids can restore macular pigment density, which declines with age. In fact, one such study of advanced macular degeneration patients who were taking high-dose lutein supplements (4 mg or more per day) experienced “normal” levels after a few months. A Harvard-led study demonstrated that eating lutein-rich foods five days per week meant subjects were eight times less likely to develop macular degeneration compared to those who consumed the same foods just once per month.
2.Quality and Specifications
5% 20% 50% HPLC
3.Product characteristics
a. Low ash, impurity, heavy metal, solvent residue.
b. Technology steadily, quality controls strictly.
c. Complete product tracing system and techinic support.

Wolfberry polysaccharide
1.Function and Application
The polysaccharide is extracted from Ningxia wolfberry (Lycium barbarum L.), which is one of the important effective compositions in this valuable fruit. It is characterized by its protein content, which is very rare in vegetarian polysaccharides. The side lateral chains of the wolfberry polysaccharide are made of rhamnose, arabinose, xylose, seminose, galactose and lucose, while the protein backbone is composed of 18 amino acids. The side chains attach to the backbone by means of Glycan-0-Der. This glycoprotein of bioactivity is high in immunological enhancement.
　　In accordance with modern medical researches, Wolfberry polysaccharide can help upgrade human immunity. It has the functions of preventing & curing cancers, strengthening hematopoiesis, nourishing liver, lowering blood pressure and promoting cell activity. No side-effect has ever been found. Wolfberry polysaccharide can control the division and differentiation of cells, and regulate the growth and consenescence of cells. Therefore, wolfberry polysaccharide has been widely used in pharmacy manufacture and healthy food processing.
2.Quality and Specifications
Polysaccharide 20%-90% UV
3.Product characteristics
a. Raw materials from Ningxia wolfberry
b. Possess diverse specifications, also could R&D the special one regarding to the customer’s own requirement.
c. The extract solvent is only with water and ethanol; which ensure the safety of the product.
d. Stable techinic with strict quality control system.
e. Low ash, impurity.
f. Complete product tracing system and techinic support.